To perform test for pyrogens on Water For Injection I. P.

D - Pharma 2nd year experiment ( HCP )


Experiment of To perform test for pyrogens on Water For Injection I. P.
experiment 3


Questions

1.Write meaning of the following terms –

1)      Isotonic solutions

2)      Hypotonic solutions

3)      Hypertonic solutions

4)      Paratonic solutions

2.Write the chemical nature of pyrogens - 

 3. Why rabbit is chosen as an animal for pyrogen testing?

 4. Why pyrogers are more dangerous if present in large volume parenterals? State two reasons

5. State the procedure for LAL'S test ?

6 What is the effect of injecting hypertonic and hiyotone solutions intravenously?

7. What is the importance of pyrogen test?  

8. State whether pyrogens are water soluble or water insoluble

9. Are pyrogens volatile or non- Volatile?

10. What does LAL stands for?

11. What are Pyrogens?

 

(Answers)

 

Q 1. Ans⇒ Meanings of following terms -

i) Isotonic Solution

A solution that hay the same Salt concentrations as cells

and blood.

2)Hypotonic solution -

A solutions that contains Pewer dissolved particles

( such as salt & other Electrolytes ) is found in normal celll & Blood .

3) Hypertonic solution –

A solution that contain more dissolved particles than is found in normal cells & Blood.

4) Paratonic Solution –

 Resulting from external stimuli paratonic plant growth.

 

Q. 2. Ans-  The nature of pyrogen are water Soluble, heat stable and filterable.

 

Q 3 Ans - Rabbits are used to perform the test Because their Body perform the test becsuse their body

temprature increases when pyrogen is introduced by the parental route

 

Q 4. Ans → Pyregens are substance that can produce

   a fever.

 

Q 7. Ans → pyrogen test is performed to cheak the presence or Absence of pyrogens in all aqueous parenterals